| |
PROTEOMICS
Definition : Dorland's Medical Dictionary defines proteomics as , " the qualitative and quantitative study of the proteome under various conditions, including protein expression, modification, localization, and function, and protein-protein interactions, as a means of understanding biological processes". To progress in clinical biotechnology, these concepts must be understood.
BOLANDCELL emphasizes the importance of this new horizon which is important for the understanding of the cell morphology, nucleus, cytocavitary network and extracellular matrix (ECM). Knowledge is needed to understand cell culturing, proliferation and cell therapy. There are many resources on the internet that readers can consult. BOLANDCELL emphasizes keywords and new language thus allowing a better understanding of cell processes and dynamics. But first you need to understand key technologies in use now: Molecular and cellular proteomics:
- Genome, genomics: protein sequencing
- Protein expression
- Transcriptone technology
- Protein microassays, immunoaffinity chromatography
- Fluoresce staining/ biomarkers
- 2-D gel electrophoresis ( 2D PAGE): allows for the identification of disease-related changes in protein expression.
- Western Blot, Immunohistochemical staining, Eliza, PCR analysis
- Autoradiography
- Image and dot analysis
- Mass spectography ( matrix-assisted laser desoption)
- Peptide mass fingerprinting
- Bioformatics
- Computer data interpretation
- Use of diagnostic markers ( disease biomarkers)
- Protein sequence analysis ( SEQUEST)
- X-ray crystallography
- Fourier transform infrared spectroscopy
- Affinity chromatography
- X-ray tomography
- Chromophore-assisted laser activation
- Application of software-based image analysis
-
COMMENT: To proceed with tissue culture a working knowledge of proteomics is necessary. A working knowledge of disease proteomics is important. Proteomic application may overcome some of the limitations of diagnosis and treatment. Profiling of protein expression in disease has not been pure but has been enhanced by the application of mass spectrometry. 
 
|
|
